| 000 | 05384nam a2200685 i 4500 | ||
|---|---|---|---|
| 001 | 8947321 | ||
| 003 | IEEE | ||
| 005 | 20200413152934.0 | ||
| 006 | m eo d | ||
| 007 | cr cn |||m|||a | ||
| 008 | 200126s2020 paua ob 000 0 eng d | ||
| 020 |
_a9781681736921 _qelectronic |
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| 020 |
_z9781681736938 _qhardcover |
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| 020 |
_z9781681736914 _qpaperback |
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| 024 | 7 |
_a10.2200/S00958ED1V01Y201910BME058 _2doi |
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| 035 | _a(CaBNVSL)thg00980002 | ||
| 035 | _a(OCoLC)1138051918 | ||
| 040 |
_aCaBNVSL _beng _erda _cCaBNVSL _dCaBNVSL |
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| 050 | 4 |
_aQP519.9.E434 _bH838 2020eb |
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| 082 | 0 | 4 |
_a541/.372 _223 |
| 100 | 1 |
_aHuang, Liang _c(Chemist), _eauthor. |
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| 245 | 1 | 0 |
_a3D electro-rotation of single cells / _cLiang Huang, Wenhui Wang. |
| 264 | 1 |
_a[San Rafael, California] : _bMorgan & Claypool, _c[2020] |
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| 300 |
_a1 PDF (xvii, 101 pages) : _billustrations (some color). |
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| 336 |
_atext _2rdacontent |
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| 337 |
_aelectronic _2isbdmedia |
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| 338 |
_aonline resource _2rdacarrier |
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| 490 | 1 |
_aSynthesis lectures on biomedical engineering, _x1930-0336 ; _v#58 |
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| 538 | _aMode of access: World Wide Web. | ||
| 538 | _aSystem requirements: Adobe Acrobat Reader. | ||
| 500 | _aPart of: Synthesis digital library of engineering and computer science. | ||
| 504 | _aIncludes bibliographical references (pages 81-100). | ||
| 505 | 0 | _a1. Introduction -- 1.1. Overview of microfluidics -- 1.2. Sample manipulation methods in microfluidic chips -- 1.3. DEP microfluidic chips -- 1.4. Research purposes and significances -- 1.5. Main content of the book | |
| 505 | 8 | _a2. Thick-electrode DEP for single-cell 3D rotation -- 2.1. Introduction -- 2.2. Progress in cell rotation manipulation -- 2.3. Thick-electrode multi-electrode chip design -- 2.4. Chip fabrication -- 2.5. Experimental setup -- 2.6. Single-cell 3D rotation experiment -- 2.7. Cellular electrical property analysis -- 2.8. Cell 3D morphology reconstruction -- 2.9. Summary | |
| 505 | 8 | _a 3. Opto-electronic integration of thick-electrode DEP microfluidic chip -- 3.1. Introduction -- 3.2. Progress in single-cell mechanical property measurement -- 3.3. Electro-rotation chip function expansion -- 3.4. Chip design and fabrication -- 3.5. Experimental setup -- 3.6. Single-cell manipulation and multi-parameter analysis experiments -- 3.7. Summary | |
| 505 | 8 | _a 4. Summary and outlook -- 4.1. Main work -- 4.2. Major innovations -- 4.3. Future prospects. | |
| 506 | _aAbstract freely available; full-text restricted to subscribers or individual document purchasers. | ||
| 510 | 0 | _aCompendex | |
| 510 | 0 | _aINSPEC | |
| 510 | 0 | _aGoogle scholar | |
| 510 | 0 | _aGoogle book search | |
| 520 | _aDielectrophoresis microfluidic chips have been widely used in various biological applications due to their advantages of convenient operation, high throughput, and low cost. However, most of the DEP microfluidic chips are based on 2D planar electrodes which have some limitations, such as electric field attenuation, small effective working regions, and weak DEP forces. In order to overcome the limitations of 2D planar electrodes, two kinds of thick-electrode DEP chips were designed to realize manipulation and multi-parameter measurement of single cells. Based on the multi-electrode structure of thick-electrode DEP, a single-cell 3D electro-rotation chip of "Armillary Sphere" was designed. The chip uses four thick electrodes and a bottom planar electrode to form an electric field chamber, which can control 3D rotation of single cells under different electric signal configurations. Electrical property measurement and 3D image reconstruction of single cells are achieved based on single-cell 3D rotation. This work overcomes the limitations of 2D planar electrodes and effectively solves the problem of unstable spatial position of single-cell samples, and provides a new platform for single-cell analysis. Based on multi-electrode structure of thick-electrode DEP, a microfluidic chip with optoelectronic integration was presented. A dual-fiber optical stretcher embedded in thick electrodes can trap and stretch a single cell while the thick electrodes are used for single-cell rotation. Stretching and rotation manipulation gives the chip the ability to simultaneously measure mechanical and electrical properties of single cells, providing a versatile platform for single-cell analysis, further extending the application of thick-electrode DEP in biological manipulation and analysis. | ||
| 530 | _aAlso available in print. | ||
| 588 | _aTitle from PDF title page (viewed on January 26, 2020). | ||
| 650 | 0 | _aElectrophoresis. | |
| 650 | 0 | _aMicrofluidics. | |
| 650 | 0 |
_aCytology _xTechnique. |
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| 650 | 0 |
_aCells _xAnalysis. |
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| 653 | _athick electrodes | ||
| 653 | _aDEP | ||
| 653 | _a3D rotation | ||
| 653 | _aoptical stretcher | ||
| 653 | _amulti-parameter measurement | ||
| 700 | 1 |
_aWang, Wenhui _c(Ph.D. in mechanical engineering), _eauthor. |
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| 776 | 0 | 8 |
_iPrint version: _z _z9781681736938 _z9781681736914 |
| 830 | 0 | _aSynthesis digital library of engineering and computer science. | |
| 830 | 0 |
_aSynthesis lectures on biomedical engineering ; _v#58. |
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| 856 | 4 | 2 |
_3Abstract with links to resource _uhttps://ieeexplore.ieee.org/servlet/opac?bknumber=8947321 |
| 856 | 4 | 0 |
_3Abstract with links to full text _uhttps://doi.org/10.2200/S00958ED1V01Y201910BME058 |
| 999 |
_c562451 _d562451 |
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