000			05480nam a2200637 i 4500
001			6813252
003			IEEE
005			20200413152855.0
006			m eo d
007			cr cn |||m|||a
008			090809s2009 caua foab 000 0 eng d
020			_a9781598299489 (electronic bk.)
020			_z9781598299472 (pbk.)
024	7		_a10.2200/S00200ED1V01Y200906SBI001 _2doi
035			_a(CaBNVSL)gtp00535453
035			_a(OCoLC)427958759
040			_aCaBNVSL _cCaBNVSL _dCaBNVSL
050		4	_aQH442 _b.K854 2009
082	0	4	_a660.65 _222
100	1		_aKuldell, Natalie.
245	1	0	_aGenome refactoring _h[electronic resource] / _cNatalie Kuldell and Neal Lerner.
260			_aSan Rafael, Calif. (1537 Fourth Street, San Rafael, CA 94901 USA) : _bMorgan & Claypool Publishers, _cc2009.
300			_a1 electronic text (xii, 66 p. : ill.) : _bdigital file.
490	1		_aSynthesis lectures on synthetic biology ; _v# 1
538			_aMode of access: World Wide Web.
538			_aSystem requirements: Adobe Acrobat reader.
500			_aPart of: Synthesis digital library of engineering and computer science.
500			_aSeries from website.
504			_aIncludes bibliographical references.
505	0		_aTools for genome engineering and synthetic biology -- Introduction -- DNA synthesis -- Abstraction as a tool for managing and understanding biological complexity -- Standards -- Genome refactoring in the service of synthetic biology -- References -- Bacteriophage as templates for refactoring -- Introduction -- T7 background -- Rebuilding T7 -- M13 background -- Rebuilding M13 -- Reflection on M13 refactoring -- References -- Methods/teaching protocols for M13 reengineering -- Introduction -- Goals and outcomes for "simple" genetic engineering of M13 -- Goals and outcomes for refactoring M13 -- References -- Writing and speaking as biological engineers -- Introduction -- Communications-intensive classes at MIT -- Learning and the development of professional identity -- Writing and speaking about synthetic biology -- Understanding students' experiences with communicating synthetic biology -- Conclusion -- References -- Summary and future directions -- Summary -- Future directions -- References -- Appendix A -- Genome engineering essay assignment (Spring 2007) -- Abstract -- Introduction -- Body: parts 1-3 -- Conclusions or summary -- Appendix B -- Part 1: rebuttal to editorial -- Part 2: mini-business plan for the registry of standard biological parts -- Appendix C -- Section 1: vocabulary -- Section 2: molecular biology.
506	1		_aAbstract freely available; full-text restricted to subscribers or individual document purchasers.
510	0		_aCompendex
510	0		_aINSPEC
510	0		_aGoogle scholar
510	0		_aGoogle book search
520	3		_aThe science of biology celebrates the discovery and understanding of biological systems that already exist in nature. In parallel, the engineering of biology must learn how to make use of our understanding of the natural world to design and build new useful biological systems. "Synthetic biology" represents one example of recent work to engineer biological systems. This emerging field aims to replace the ad hoc process of assembling biological systems by primarily developing tools to assemble reliable-but-complex living organisms from standard components that can later be reused in new combination. The focus of this book is "genome refactoring," one of several approaches to manage the complexity of a biological system in which the goal is to redesign the genetic elements that encode a living form--preserving the function of that form but encoding it with a genome far easier to study and extend. This book presents genome refactoring in two ways: as an important aspect of the emerging field of synthetic biology and as a powerful teaching tool to train would be professionals in the subject. Chapters focus on the overarching goals of synthetic biology and their alignment with the motivations and achievements in genome engineering; the engineering frameworks of refactoring, including genome synthesis, standardization of biological parts, and abstraction; a detailed description of the bacteriophages that have been refactored up to this point; and the methods of refactoring and contexts for that work drawn from the bacteriophage M13. Overall, these examples offer readers the potential for synthetic biology and the areas in need of further research. If successful, synthetic biology and genome refactoring could address any number of persistent societal needs, including sustainable energy, affordable and effective medicine, and green manufacturing practices.
530			_aAlso available in print.
588			_aTitle from PDF t.p. (viewed on August 9, 2009).
650		0	_aGenetic engineering.
650		0	_aBacteriophages _xGenetics.
650		0	_aViral genomes.
690			_aSynthetic biology
690			_aGenome refactoring
690			_aAbstraction
690			_aStandardization
690			_aModularity
690			_aDNA synthesis
690			_aBacteriophage
690			_aT7
690			_aM13
690			_aGenetic parts
700	1		_aLerner, Neal.
730	0		_aSynthesis digital library of engineering and computer science.
830		0	_aSynthesis lectures on synthetic biology ; _v# 1.
856	4	2	_3Abstract with links to resource _uhttp://ieeexplore.ieee.org/servlet/opac?bknumber=6813252
999			_c561697 _d561697