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001			978-1-4020-6316-9
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020			_a9781402063169 _9978-1-4020-6316-9
024	7		_a10.1007/978-1-4020-6316-9 _2doi
050		4	_aQD901-999
072		7	_aPHFC _2bicssc
072		7	_aSCI016000 _2bisacsh
082	0	4	_a548 _223
245	1	0	_aEvolving Methods for Macromolecular Crystallography _h[electronic resource] : _bThe Structural Path to the Understanding of the Mechanismof Action of CBRN Agents / _cedited by Randy J. Read, Joel L. Sussman.
246	3		_aProceedings of the NATO Advanced Study Institute on Evolving Methods for Macromolecular Crystallography: The Structural Path to the Understanding of the Mechanism of Action of CBRN agents, Erice, Italy, 19-28 May 2005
264		1	_aDordrecht : _bSpringer Netherlands, _c2007.
300			_aVIII, 192 p. _bonline resource.
336			_atext _btxt _2rdacontent
337			_acomputer _bc _2rdamedia
338			_aonline resource _bcr _2rdacarrier
347			_atext file _bPDF _2rda
490	1		_aNATO Science Series, _x1568-2609 ; _v245
505	0		_aSucceeding with seeding: some practical advice -- Expression, purification, and crystallisationof membrane proteins -- Macromolecular cryo-crystallography -- Processing diffraction data with mosflm -- Sad phasing: basic concepts and high-throughput -- Likelihood-based experimental phasing in phaser -- Stochastic molecular replacement -- Likelihood-based molecular replacement in phaser -- Automated structure determination with phenix -- Density Modification In Main -- Ab initio phasing starting from low resolution -- Structural genomics of mycobacterium tuberculosis: a search for function and new drug targets -- Three-dimensional domain swapping and its relevance to conformational diseases -- Structural bioinformatics: from protein structure to function -- Single-particle imaging.
520			_aThis volume comprises papers presented at the 2005 edition of the “Crystallography of Molecular Biology” courses that have been held since 1976 at the Ettore Majorana Centre for Scientific Culture in Erice, Italy. This series of courses is renowned for bringing leaders in the field of macromo- cular crystallography together with highly motivated students, in a beautiful and intimate location that encourages people to interact. The warm and informal atmosphere at these Erice conferences, especially these on cryst- lography, has helped to foster long-term scientific interactions and new int- national friendships that have often lasted for the lifetime of the scientists. The course was financed by NATO as an Advanced Study Institute and by the European Commission as a EuroSummerSchool. The papers span the breadth of material presented in the course, which emphasized the practical aspects of modern macromolecular crystallography and its applications. One must start with crystals: Bergfors showed how to improve initial crystals through seeding, while Byrne discussed the difficult problem of crystallizing membrane proteins. The collection of optimal diffraction data requires both careful preparation of cryo-cooled crystals (Garman) and proper processing of the diffraction images (Leslie). To obtain images of electron density, one needs estimates of the phases of the diffracted spots. Sheldrick presented the background to the sing- wavelength anomalous diffraction (SAD) method, which has been gaining popularity, and McCoy discussed the basis of modern maximum likelihood methods for treating information in experimental phasing.
650		0	_aPhysics.
650		0	_aBioinformatics.
650		0	_aComputational biology.
650		0	_aCrystallography.
650		0	_aBiophysics.
650		0	_aBiological physics.
650	1	4	_aPhysics.
650	2	4	_aCrystallography.
650	2	4	_aBiophysics and Biological Physics.
650	2	4	_aBioinformatics.
650	2	4	_aComputer Appl. in Life Sciences.
700	1		_aRead, Randy J. _eeditor.
700	1		_aSussman, Joel L. _eeditor.
710	2		_aSpringerLink (Online service)
773	0		_tSpringer eBooks
776	0	8	_iPrinted edition: _z9781402063145
830		0	_aNATO Science Series, _x1568-2609 ; _v245
856	4	0	_uhttp://dx.doi.org/10.1007/978-1-4020-6316-9
912			_aZDB-2-PHA
950			_aPhysics and Astronomy (Springer-11651)
999			_c507873 _d507873