000 03737nam a22004815i 4500
001 978-1-4020-3500-5
003 DE-He213
005 20161121230942.0
007 cr nn 008mamaa
008 100301s2006 ne | s |||| 0|eng d
020 _a9781402035005
_9978-1-4020-3500-5
024 7 _a10.1007/1-4020-3500-4
_2doi
050 4 _aQH505
072 7 _aPHVN
_2bicssc
072 7 _aPHVD
_2bicssc
072 7 _aSCI009000
_2bisacsh
082 0 4 _a571.4
_223
100 1 _aRule, Gordon S.
_eauthor.
245 1 0 _aFundamentals of Protein NMR Spectroscopy
_h[electronic resource] /
_cby Gordon S. Rule, T. Kevin Hitchens.
264 1 _aDordrecht :
_bSpringer Netherlands,
_c2006.
300 _aXXVI, 532 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aFocus on Structural Biology,
_x1571-4853 ;
_v5
505 0 _aNMR Spectroscopy -- Practical Aspects of Acquiring NMR Spectra -- to Signal Processing -- Quantum Mechanical Description of NMR -- Quantum Mechanical Description of a One Pulse Experiment -- The Density Matrix & Product Operators -- Scalar Coupling -- Coupled Spins: Density Matrix and Product Operator Formalism -- Two Dimensional Homonuclear J-Correlated Spectroscopy -- Two Dimensional Heteronuclear J-Correlated Spectroscopy -- Coherence Editing: Pulsed-Field Gradients and Phase Cycling -- Quadrature Detection in Multi-Dimensional NMR Spectroscopy -- Resonance Assignments: Homonuclear Methods -- Resonance Assignments: Heteronuclear Methods -- Practical Aspects of N-Dimensional Data Acquisition and Processing -- Dipolar Coupling -- Protein Structure Determination -- Exchange Processes -- Nuclear Spin Relaxation and Molecular Dynamics.
520 _aNMR spectroscopy has proven to be a powerful technique to study the structure and dynamics of biological macromolecules. Fundamentals of Protein NMR Spectroscopy is a comprehensive textbook that guides the reader from a basic understanding of the phenomenological properties of magnetic resonance to the application and interpretation of modern multi-dimensional NMR experiments on 15N/13C-labeled proteins. Beginning with elementary quantum mechanics, a set of practical rules is presented and used to describe many commonly employed multi-dimensional, multi-nuclear NMR pulse sequences. A modular analysis of NMR pulse sequence building blocks also provides a basis for understanding and developing novel pulse programs. This text not only covers topics from chemical shift assignment to protein structure refinement, as well as the analysis of protein dynamics and chemical kinetics, but also provides a practical guide to many aspects of modern spectrometer hardware, sample preparation, experimental set-up, and data processing. End of chapter exercises are included to emphasize important concepts. Fundamentals of Protein NMR Spectroscopy not only offer students a systematic, in-depth, understanding of modern NMR spectroscopy and its application to biomolecular systems, but will also be a useful reference for the experienced investigator.
650 0 _aPhysics.
650 0 _aBiophysics.
650 0 _aBiological physics.
650 1 4 _aPhysics.
650 2 4 _aBiophysics and Biological Physics.
650 2 4 _aBiomedicine general.
700 1 _aHitchens, T. Kevin.
_eauthor.
710 2 _aSpringerLink (Online service)
773 0 _tSpringer eBooks
776 0 8 _iPrinted edition:
_z9781402034992
830 0 _aFocus on Structural Biology,
_x1571-4853 ;
_v5
856 4 0 _uhttp://dx.doi.org/10.1007/1-4020-3500-4
912 _aZDB-2-PHA
950 _aPhysics and Astronomy (Springer-11651)
999 _c506363
_d506363