000			03425nam a22004935i 4500
001			978-1-59745-228-1
003			DE-He213
005			20161121230803.0
007			cr nn 008mamaa
008			100301s2007 xxu| s |||| 0|eng d
020			_a9781597452281 _9978-1-59745-228-1
024	7		_a10.1007/978-1-59745-228-1 _2doi
050		4	_aQH345
050		4	_aQD415-436
072		7	_aPSB _2bicssc
072		7	_aSCI007000 _2bisacsh
082	0	4	_a572 _223
245	1	0	_aRegulation of Gene Expression _h[electronic resource] : _bMolecular Mechanisms / _cedited by Gary H. Perdew, John P. Vanden Heuvel, Jeffrey M. Peters.
264		1	_aTotowa, NJ : _bHumana Press, _c2007.
300			_aIX, 333 p. _bonline resource.
336			_atext _btxt _2rdacontent
337			_acomputer _bc _2rdamedia
338			_aonline resource _bcr _2rdacarrier
347			_atext file _bPDF _2rda
505	0		_aGene Expression Control At The Mrna Level -- Overview -- Messenger RNA Accumulation -- Transcript Profiling -- Transcriptional Regulation of Gene Expression -- Posttranscriptional Processing of Messenger RNA -- Regulation Of Protein Levels And Transcription Factor Function -- Overview -- Protein Synthesis and Turnover -- Proteomics -- Determination of Protein-Protein Interactions and the Motifs That Mediate Them -- Posttranslational Modifications -- Dissection of Signaling Pathways -- Use Of Transgenic And Knockout Mice To Study Gene Regulation -- Overview -- Production of Transgenic Animals -- Knockout Animals -- Isolation of Cells and Cell Lines From Transgenic and Knockout Animals.
520			_aRegulation of Gene Expression: Molecular Mechanisms presents a comprehensive overview of methods and approaches for characterizing mechanisms of gene regulation. The text is appropriate both as a graduate textbook and a standard laboratory reference and provides the essential groundwork for an advanced understanding of the various mechanisms that may result in altered activity of a specific cell protein. Each of three sections explores mechanisms of gene regulation and expression, and presents methods and protocols for achieving specific experimental goals. Part I focuses on approaches for studying control of mRNA expression and determining target genes for a given transcription copy. Part II outlines the methods for determining how proteins can regulate each other by mediating synthesis, degradation, protein-protein interactions, and posttranslational modification. Part III explores how gene targeting techniques in mice can provide insight into protein function. This volume provides a clear, concise overview of the protocols and techniques used to examine chemically or disease-mediated alterations in gene expression in mammalian systems.
650		0	_aLife sciences.
650		0	_aHuman genetics.
650		0	_aPharmacology.
650		0	_aBiochemistry.
650	1	4	_aLife Sciences.
650	2	4	_aBiochemistry, general.
650	2	4	_aPharmacology/Toxicology.
650	2	4	_aHuman Genetics.
700	1		_aPerdew, Gary H. _eeditor.
700	1		_aHeuvel, John P. Vanden. _eeditor.
700	1		_aPeters, Jeffrey M. _eeditor.
710	2		_aSpringerLink (Online service)
773	0		_tSpringer eBooks
776	0	8	_iPrinted edition: _z9781588292650
856	4	0	_uhttp://dx.doi.org/10.1007/978-1-59745-228-1
912			_aZDB-2-SBL
950			_aBiomedical and Life Sciences (Springer-11642)
999			_c503958 _d503958