000 03886nam a22005175i 4500
001 978-0-387-72972-5
003 DE-He213
005 20161121230751.0
007 cr nn 008mamaa
008 100301s2008 xxu| s |||| 0|eng d
020 _a9780387729725
_9978-0-387-72972-5
024 7 _a10.1007/978-0-387-72972-5
_2doi
050 4 _aQH201-278.5
072 7 _aPS
_2bicssc
072 7 _aPDND
_2bicssc
072 7 _aSCI086000
_2bisacsh
072 7 _aSCI023000
_2bisacsh
082 0 4 _a570.282
_223
245 1 0 _aBiological Low-Voltage Scanning Electron Microscopy
_h[electronic resource] /
_cedited by Heide Schatten, James B. Pawley.
264 1 _aNew York, NY :
_bSpringer New York,
_c2008.
300 _aX, 317 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
505 0 _aThe Early Development of the Scanning Electron Microscope -- LVSEM for Biology -- The Aberration-Corrected SEM -- Noise and Its Effects on the Low-Voltage SEM -- High-Resolution, Low Voltage, Field-Emission Scanning Electron Microscopy (HRLVFESEM) Applications for Cell Biology and Specimen Preparation Protocols -- Molecular Labeling for Correlative Microscopy: LM, LVSEM, TEM, EF-TEM and HVEM -- Low kV and Video-Rate, Beam-Tilt Stereo for Viewing Live-Time Experiments in the SEM -- Cryo-SEM of Chemically Fixed Animal Cells -- High-Resolution and Low-Voltage SEM of Plant Cells -- High-Resolution Cryoscanning Electron Microscopy of Biological Samples -- Developments in Instrumentation for Microanalysis in Low-Voltage Scanning Electron Microscopy.
520 _aField-emission, low-voltage scanning electron microscopy (LVSEM) is a field that has grown tremendously in recent years because is offers the optimal method for viewing complex surfaces at high resolution and in three dimensions. However, even though the instrumentation required to get good results at low beam voltage has become increasingly available, there has been a lag in its application to biological specimens. What seemed to be missing was volume that combined both the theory and practice of using this equipment in an optimal manner with a thorough treatment of biological specimen preparation. Biological Low-Voltage Scanning Electron Microscopy is the first book to address both of these aspects of biological LVSEM. After providing a thorough description of the unique advantages and the operating constraints related to operating a scanning electron microscope at low beam voltage, the remainder of book focuses on the the best way to image all types of plant and animal cells and covers specimens that range from macromolecules to the surfaces revealed by de-embedding resin-embedded samples. Advanced specimen preparation techniques such as cryo-LVSEM, and immuno-gold-LVSEM are fully covered, as is x-ray microanalysis at low beam voltage and live-time stereo imaging. The preparative protocols provided represent the distilled essence of the experience of a group of world-renowned authors who have, for many decades, been instrumental in developing and applying new approaches to LVSEM to support their own biological research.
650 0 _aLife sciences.
650 0 _aCell biology.
650 0 _aMicrobiology.
650 0 _aZoology.
650 0 _aMicroscopy.
650 1 4 _aLife Sciences.
650 2 4 _aBiological Microscopy.
650 2 4 _aZoology.
650 2 4 _aCell Biology.
650 2 4 _aMicrobiology.
700 1 _aSchatten, Heide.
_eeditor.
700 1 _aPawley, James B.
_eeditor.
710 2 _aSpringerLink (Online service)
773 0 _tSpringer eBooks
776 0 8 _iPrinted edition:
_z9780387729701
856 4 0 _uhttp://dx.doi.org/10.1007/978-0-387-72972-5
912 _aZDB-2-SBL
950 _aBiomedical and Life Sciences (Springer-11642)
999 _c503669
_d503669