000			03294nam a22005415i 4500
001			978-3-540-28489-5
003			DE-He213
005			20161121230705.0
007			cr nn 008mamaa
008			100301s2006 gw | s |||| 0|eng d
020			_a9783540284895 _9978-3-540-28489-5
024	7		_a10.1007/978-3-540-28489-5 _2doi
050		4	_aQH432
072		7	_aPSVH _2bicssc
072		7	_aPSAK1 _2bicssc
072		7	_aSCI070000 _2bisacsh
072		7	_aSCI029000 _2bisacsh
082	0	4	_a591.35 _223
245	1	0	_aMammalian and Avian Transgenesis — New Approaches _h[electronic resource] / _cedited by Shirley Pease, Carlos Lois.
264		1	_aBerlin, Heidelberg : _bSpringer Berlin Heidelberg, _c2006.
300			_aXX, 281 p. 48 illus., 12 illus. in color. _bonline resource.
336			_atext _btxt _2rdacontent
337			_acomputer _bc _2rdamedia
338			_aonline resource _bcr _2rdacarrier
347			_atext file _bPDF _2rda
490	1		_aPrinciples and Practice
505	0		_aGeneration of Transgenic Animals Using Lentiviral Vectors -- Intracytoplasmic Sperm Injection (ICSI) in the Mouse -- Generation of Embryonic Stem (ES) Cell-Derived Embryos and Mice by Tetraploid-Embryo Complementation -- Cloning The Laboratory Mouse by Nuclear Transfer -- Large Insert Transgenesis -- Regional and Temporal Control of Genetic Manipulation in the Mouse -- High Resolution Gene Expression Analysis in Mice Using Genetically Inserted Reporter Genes -- Nuclear Transfer in the Cow -- Production of Transgenic Pigs by DNA Microinjection -- Production of Transgenic Birds Using Lentiviral Vectors -- Ancillary Techniques.
520			_aMammalian and Avian Transgenesis presents a collection of novel methods for the production of a wide range of transgenic animals. The manual focuses largely on mice, but also contains protocols for successful transgenesis in rats, cows, pigs and birds. The manual provides detailed, step-by-step protocols covering all aspects of the production of transgenic animals, including the use of lentiviral vectors in gene transfer, intracytoplasmic sperm injection, nuclear transfer, large insert transgenesis, conditional gene expression systems, the use of reporter genes in transgenesis and transgenesis in large animals and birds. The text is supplemented by superb color photos. While the focus is on newly established techniques, the fundamental methods of transgenesis are also covered for those new to the field. Thus this manual is perfectly suited for those wishing to adopt new technologies in transgenesis.
650		0	_aLife sciences.
650		0	_aMolecular biology.
650		0	_aCell biology.
650		0	_aDevelopmental biology.
650		0	_aAnimal genetics.
650	1	4	_aLife Sciences.
650	2	4	_aAnimal Genetics and Genomics.
650	2	4	_aCell Biology.
650	2	4	_aDevelopmental Biology.
650	2	4	_aMolecular Medicine.
700	1		_aPease, Shirley. _eeditor.
700	1		_aLois, Carlos. _eeditor.
710	2		_aSpringerLink (Online service)
773	0		_tSpringer eBooks
776	0	8	_iPrinted edition: _z9783540284154
830		0	_aPrinciples and Practice
856	4	0	_uhttp://dx.doi.org/10.1007/978-3-540-28489-5
912			_aZDB-2-SBL
950			_aBiomedical and Life Sciences (Springer-11642)
999			_c502513 _d502513