000			05340nam a22005775i 4500
001			978-1-4020-3616-3
003			DE-He213
005			20161121230657.0
007			cr nn 008mamaa
008			100301s2005 ne | s |||| 0|eng d
020			_a9781402036163 _9978-1-4020-3616-3
024	7		_a10.1007/1-4020-3616-7 _2doi
050		4	_aQH301-705
072		7	_aPSA _2bicssc
072		7	_aSCI086000 _2bisacsh
082	0	4	_a570 _223
245	1	0	_aFrom Cells to Proteins: Imaging Nature across Dimensions _h[electronic resource] : _bProceedings of the NATO Advanced Study Institute on From Cells to Proteins: Imaging Nature across Dimensions Pisa, Italy 12–23 September 2004 / _cedited by Valtere Evangelista, Laura Barsanti, Vincenzo Passarelli, Paolo Gualtieri.
264		1	_aDordrecht : _bSpringer Netherlands, _c2005.
300			_aXII, 479 p. _bonline resource.
336			_atext _btxt _2rdacontent
337			_acomputer _bc _2rdamedia
338			_aonline resource _bcr _2rdacarrier
347			_atext file _bPDF _2rda
490	1		_aNATO Security through Science Series, Series B: Physics and Biophysics
505	0		_aOptical Microscopy for Cell Imaging -- to Transmission and Scanning Electron Microscopy -- Potential and Limitation of Cytochemistry -- Cryotechniques for Electron Microscopy: A Minireview -- Cell Membrane Specializations as Revealed by the Freeze-Fracture Technique -- Imaging, Measuring and Manipulating Biological Matter from the Millimeter to Nanometer Scale -- Inside the Small Length and Energy Scales of the World of the Individual Biological Molecule -- Scanning Probe Microscopy across Dimensions -- Imaging Cells Using Soft X-Rays -- From Microscopy to Nanoscopy: How to Get and Read Optical Data at Single Molecule Level Using Confocal and Two-Photon Excitation Microscopy -- Fluorescence Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) -- Quantum Dots, a New Tool for Real-Time in Vivo Imaging -- Image Acquisition and its Automation in Fluorescence Microscopy -- Stereological and Digital Methods for Estimating Geometrical Characteristics of Biological Structures Using Confocal Microscopy -- Wavelength as the Fourth Dimension in Light Microscopy -- Time as the Fifth Dimension in Microscopy -- Image Deconvolution -- Image Formation in Fluorescence Microscopy -- Atomic Force Microscopy Study on the Pellicle of the Alga Euglena gracilis -- Atomic Force Microscopy Study of Pili in the Cyanobacterium Synechocystis SP. PCC 6803 -- Changes of Algae Protein Complex under pH Effect -- Leaf Fluorescence as Diagnostic Tool for Monitoring Vegetation -- Dynamic Holography for Study of Nonlinear Optical Processes in Biological Photoreceptor Molecule -- “Seeing” Lipid Membranes by Solid-State NMR -- Visuomotor Coordination in Behaviour of Pigeons Following Post-Hatching Monocular Experience: An Image Analysis Study.
520			_aHow deep we can see inside Nature's smallest secrets? Will it be possible some day in the near future to investigate living structures at atomic level? This area of study is very interdisciplinary, since it applies the principles and the techniques of biology, physics, chemistry, mathematics, and engineering to elucidate the structures of biological macromolecules, of supramolecular structures, organelles, and cells. This book offers updated information on how much information we are able to obtain in the exploration of the inner details of biological specimens in their native structure and composition. The book deals with the implementation of laser beam and stage scanning systems incorporating confocal optics or multiphoton microscopy; the advent of new electro-optical detectors with great sensitivity, linearity, and dynamic range; the possibility of 2D fast image enhancement, reconstruction, restoration, analysis and 3D display, and the application of luminescence techniques (FLIMT, FRET combined with the use of quantum dots), which gives the possibility to investigate the chemical and molecular spatio-temporal organization of life processes; Electron Microscopy and Scanning Force Microscopy (SFM), are also presented, which has opened completely new perspectives for analyzing the surface topography of biological matter in its aqueous environment at a resolution comparable to that achieved by EM.
650		0	_aLife sciences.
650		0	_aCell biology.
650		0	_aBioinformatics.
650		0	_aComputational biology.
650		0	_aMicroscopy.
650		0	_aPhysical measurements.
650		0	_aMeasurement.
650	1	4	_aLife Sciences.
650	2	4	_aLife Sciences, general.
650	2	4	_aCell Biology.
650	2	4	_aComputer Appl. in Life Sciences.
650	2	4	_aBiological Microscopy.
650	2	4	_aMeasurement Science and Instrumentation.
700	1		_aEvangelista, Valtere. _eeditor.
700	1		_aBarsanti, Laura. _eeditor.
700	1		_aPassarelli, Vincenzo. _eeditor.
700	1		_aGualtieri, Paolo. _eeditor.
710	2		_aSpringerLink (Online service)
773	0		_tSpringer eBooks
776	0	8	_iPrinted edition: _z9781402036149
830		0	_aNATO Security through Science Series, Series B: Physics and Biophysics
856	4	0	_uhttp://dx.doi.org/10.1007/1-4020-3616-7
912			_aZDB-2-SBL
950			_aBiomedical and Life Sciences (Springer-11642)
999			_c502308 _d502308