000			03277nam a22004215i 4500
001			978-1-4020-3404-6
003			DE-He213
005			20161121230656.0
007			cr nn 008mamaa
008			100301s2005 ne | s |||| 0|eng d
020			_a9781402034046 _9978-1-4020-3404-6
024	7		_a10.1007/1-4020-3404-0 _2doi
050		4	_aR-RZ
072		7	_aMBGR _2bicssc
072		7	_aMED000000 _2bisacsh
082	0	4	_a610 _223
100	1		_aViljoen, Gerrit J. _eauthor.
245	1	0	_aMolecular Diagnostic PCR Handbook _h[electronic resource] / _cby Gerrit J. Viljoen, Louis H. Nel, John R. Crowther.
264		1	_aDordrecht : _bSpringer Netherlands, _c2005.
300			_aXVIII, 308 p. _bonline resource.
336			_atext _btxt _2rdacontent
337			_acomputer _bc _2rdamedia
338			_aonline resource _bcr _2rdacarrier
347			_atext file _bPDF _2rda
505	0		_aBackground -- PCR — The Basic Reaction -- Amplicon Detection, Analysis and PCR Evaluation -- Limitations and Troubleshooting -- Specific Types of PCR Assays -- The PCR Laboratory -- Disease Diagnosis Using PCR Specific Procedures for Important Veterinary Pathogens.
520			_aPREFACE The Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture is involved in agricultural research and development and assists Member States of FAO and IAEA in improving strategies to ensure food security through the use of nuclear techniques and related biotechnologies, where such techniques have a valuable and often unique role. In particular, molecular diagnostic methods have rapidly evolved in the past twenty years, since the advent of the Polymerase Chain Reaction (PCR). They are used in a wide range of agricultural areas such as, improving soil and water management; producing better crop varieties; diagnosing plant and animal diseases; controlling insect pests and improving food quality and safety. The uses of nucleic acid-directed methods have increased significantly in the past five years and have made important contributions to disease control country programmes for improving national and international trade. These developments include the more routine use of PCR as a diagnostic tool in veterinary diagnostic laboratories. However, there are many problems associated with the transfer and particularly, the application of this technology. These include lack of consideration of: the establishment of quality-assured procedures, the required set-up of the laboratory and the proper training of staff. This can lead to a situation where results are not assured. This book gives a comprehensive account of the practical aspects of PCR and strong consideration is given to ensure its optimal use in a laboratory environment. This includes the setting-up of a PCR laboratory; Good Laboratory Practice and standardised of PCR protocols.
650		0	_aMedicine.
650	1	4	_aBiomedicine.
650	2	4	_aBiomedicine general.
700	1		_aNel, Louis H. _eauthor.
700	1		_aCrowther, John R. _eauthor.
710	2		_aSpringerLink (Online service)
773	0		_tSpringer eBooks
776	0	8	_iPrinted edition: _z9781402034039
856	4	0	_uhttp://dx.doi.org/10.1007/1-4020-3404-0
912			_aZDB-2-SBL
950			_aBiomedical and Life Sciences (Springer-11642)
999			_c502298 _d502298