000 03140nam a22003975i 4500
001 978-1-4020-3319-3
003 DE-He213
005 20161121230656.0
007 cr nn 008mamaa
008 100301s2005 ne | s |||| 0|eng d
020 _a9781402033193
_9978-1-4020-3319-3
024 7 _a10.1007/1-4020-3319-2
_2doi
050 4 _aR-RZ
072 7 _aMBGR
_2bicssc
072 7 _aMED000000
_2bisacsh
082 0 4 _a610
_223
245 1 0 _aAcceleration and Improvement of Protein Identification by Mass Spectrometry
_h[electronic resource] /
_cedited by Willy Vincent Bienvenut.
264 1 _aDordrecht :
_bSpringer Netherlands,
_c2005.
300 _aXXII, 298 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
505 0 _aPreface -- Acknowledgements -- List of Contributors -- 1: Introduction: Proteins analysis using mass spectrometry -- 2: Molecular scanner development: Toward clinical molecular scanner for proteome research: Parallel protein chemical processing before and during western-blot -- 3: Quantitation during electroblotting step: Enhanced protein recovery after electrotransfer using square wave alternating voltage -- 4: Signal traitment and virtual imaging (1/2): A molecular scanner to highly automated research and to display proteome images -- 5: Signal traitment and virtual imaging (2/2): Visualization and analysis of molecular scanner peptide mass spectra -- 6: Improvement in the peptide mass fingerprint protein identification (1/2): Hydrogen/deuterium exchange for higher specificity of protein identification by peptide mass fingerprinting -- 7: Improvement in the peptide mass fingerprint protein identification (2/2): MALDI-MS/MS with high resolution and sensitivity for identification and characterization of proteins -- 8: Proteomic and mass spectrometry: Some aspects and recent developments -- 9: Conclusions and perspectives -- Appendix -- Abbreviations used in this book -- Abbreviations for usual amino acids and chemical constants -- Index.
520 _aAt present where protein identification and characterisation using mass spectrometry is a method of choice, this book is presenting a review of basic proteomic techniques. The second part of the book is related to the novel high throughput protein identification technique called the 'molecular scanner'. Several protein identification techniques are described, especially the peptide mass fingerprint with MALDI-MS based method. E.g. ionisation process, matrix available, signal reproducibility and suppression effect, as well as date treatment for protein identification using bioinformatics tools.
650 0 _aMedicine.
650 1 4 _aBiomedicine.
650 2 4 _aBiomedicine general.
700 1 _aBienvenut, Willy Vincent.
_eeditor.
710 2 _aSpringerLink (Online service)
773 0 _tSpringer eBooks
776 0 8 _iPrinted edition:
_z9781402033186
856 4 0 _uhttp://dx.doi.org/10.1007/1-4020-3319-2
912 _aZDB-2-SBL
950 _aBiomedical and Life Sciences (Springer-11642)
999 _c502293
_d502293