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System requirements: Adobe Acrobat Reader.

Part of: Synthesis digital library of engineering and computer science.

Includes bibliographical references (pages 81-100).

1. Introduction -- 1.1. Overview of microfluidics -- 1.2. Sample manipulation methods in microfluidic chips -- 1.3. DEP microfluidic chips -- 1.4. Research purposes and significances -- 1.5. Main content of the book

2. Thick-electrode DEP for single-cell 3D rotation -- 2.1. Introduction -- 2.2. Progress in cell rotation manipulation -- 2.3. Thick-electrode multi-electrode chip design -- 2.4. Chip fabrication -- 2.5. Experimental setup -- 2.6. Single-cell 3D rotation experiment -- 2.7. Cellular electrical property analysis -- 2.8. Cell 3D morphology reconstruction -- 2.9. Summary

3. Opto-electronic integration of thick-electrode DEP microfluidic chip -- 3.1. Introduction -- 3.2. Progress in single-cell mechanical property measurement -- 3.3. Electro-rotation chip function expansion -- 3.4. Chip design and fabrication -- 3.5. Experimental setup -- 3.6. Single-cell manipulation and multi-parameter analysis experiments -- 3.7. Summary

4. Summary and outlook -- 4.1. Main work -- 4.2. Major innovations -- 4.3. Future prospects.

Abstract freely available; full-text restricted to subscribers or individual document purchasers.

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Dielectrophoresis microfluidic chips have been widely used in various biological applications due to their advantages of convenient operation, high throughput, and low cost. However, most of the DEP microfluidic chips are based on 2D planar electrodes which have some limitations, such as electric field attenuation, small effective working regions, and weak DEP forces. In order to overcome the limitations of 2D planar electrodes, two kinds of thick-electrode DEP chips were designed to realize manipulation and multi-parameter measurement of single cells. Based on the multi-electrode structure of thick-electrode DEP, a single-cell 3D electro-rotation chip of "Armillary Sphere" was designed. The chip uses four thick electrodes and a bottom planar electrode to form an electric field chamber, which can control 3D rotation of single cells under different electric signal configurations. Electrical property measurement and 3D image reconstruction of single cells are achieved based on single-cell 3D rotation. This work overcomes the limitations of 2D planar electrodes and effectively solves the problem of unstable spatial position of single-cell samples, and provides a new platform for single-cell analysis. Based on multi-electrode structure of thick-electrode DEP, a microfluidic chip with optoelectronic integration was presented. A dual-fiber optical stretcher embedded in thick electrodes can trap and stretch a single cell while the thick electrodes are used for single-cell rotation. Stretching and rotation manipulation gives the chip the ability to simultaneously measure mechanical and electrical properties of single cells, providing a versatile platform for single-cell analysis, further extending the application of thick-electrode DEP in biological manipulation and analysis.

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Title from PDF title page (viewed on January 26, 2020).